2 edition of Regeneration, vernalization and flowering in Lunaria annua L. in vivo and in vitro. found in the catalog.
Regeneration, vernalization and flowering in Lunaria annua L. in vivo and in vitro.
Pierik, R. L. M.
|Statement||[By] R. L. M. Pierik.|
|Series||Mededelingen Landbouwhogeschool Wageningen, Nederland, 67-6, Publication 295, Laboratorium voor Tuinbouwplantenteelt, Landbouwhogeschool, Wageningen, The Netherlands., Mededelingen (Landbouwhogeschool Wageningen) ;, 67-6.|
|LC Classifications||S239.S2 W3 deel 67-6|
|The Physical Object|
|Number of Pages||71|
|LC Control Number||68140055|
The frequency of shoot regeneration and mean number of shoots per explant was determined after 12 weeks. Rooting of the regenerated shoots In vitro regenerated shoots that originated from immature embryos were rooted on 1 mg/L indolebutyric acid (IBA) alone or in combinations with or mg/L . Glucosinolates (GSLs) from Lunaria annua L. seeds were analyzed qualitatively and quantitatively by their desulfo counterparts using UHPLC-DAD-MS/MS technique and by their volatile breakdown products, isothiocyanates (ITCs), using GC-MS technique. GSL breakdown products were obtained by conventional techniques (hydrodistillation in a Clevenger type apparatus (HD), CH2Cl2 extraction after.
influence the organogenic response of in vitro cultures in different plant species. Per cent regeneration, number of shoots per explants and per cent elongation was maximum in hybrid Indra as compared to Bharat, on MS medium supplemented with mg/l zeatin along with mg/l GA 3 and mg/l kinetin along with mg/l GA 3. Full text of "The genera of Alysseae (Cruciferae: Brassicaceae) in the southeastern United States." See other formats.
Effect of moisture content on in vitro regeneration of embryonicaxis explants of cowpea (Vigna unguiculata L.) To see the effect of ultra desiccation on seed survival, cowpea (Vigna unguiculata L.) seeds were conditioned to various moisture contents ranging from %. The use of genetic engineering for plant improvement permits the introduction of useful agronomic traits without altering the other desirable features of the cultivar. However, it is pre-requisite to have an efficient in vitro regeneration system. To date, the regeneration of grape plants has been obtained by both organogenesis and embryogenesis.
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REGENERATION, VERNALIZATION AND FLOWERING IN LUNARIA ANNUA L. IN VIVO AND IN VITRO Regeneratie, vernalisatie en bloei in Lunaria annua L. in vivo en in vitro R. PIERIK PublicationLaboratorium voor Tuinbouwplantenteelt, Landbouwhogeschool, Wageningen, The Netherlands (Received ) H.
VEENMAN & ZONEN Regeneration. - WAGENINGEN - Title: Regeneration, vernalization and flowering in Lunaria annua L. in vivo and in vitro: Author: Pierik, R.L.M.
Degree grantor: Wageningen UniversityCited by: An annual flowering mutant was obtained with ethyl methane sulphonate. Experiments with shoot and leaf cuttings showed that juvenility was not a character of the whole : R.L.M. Pierik. REGENERATION, VERNALIZATION AND FLOWERING IN LUNARIA ANNUA L.
IN VIVO AND IN VITRO Regenerate, vernalisatie en bloei in Lunaria annua L. in vivo en in vitro R. ^ ^ ^ ".»** NN BiALKJ IBfcMI D£S kBDSOUWHOGESCBO©.
- rJCited by: shoot regeneration and flowering of Sesamum indicum L. In vitro flowering has been reported as a rare p rocess o f impo rtance in crop plants, mainly its high genetic purity (Stephen and Jayabalan. In vitro flowering and effective micropropagation protocol were studied in Swertia chirayita, an important medicinal plant using axillary bud Murashige and Skoog's medium (MS) supplemented with benzyl amino purine (BAP) mg L −1 and adenine sulfate mg L −1 was found optimum for production of multiple shoots.
In the present study, incubation of flowering cultures on BAP. Lunaria annua is a biennial cruciferous oil seed crop. The seeds contain 30–35% oil, which consists of 67% long chain fatty acids (44% erucic acid, C, and 23% nervonic acid, C).
Introduction. Lunaria annua is a biennial cruciferous oil seed a is grown as an ornamental plant in many temperate countries of Europe and Northern America, but originates from south eastern Europe and western Asia (Baily, ).In ornamental gardens, plants shed their seeds late in autumn and seeds germinate in the very early spring.
Lunaria annua is a biennial cruciferous oil seed crop. The seeds contain % oil, which consists of 67% long chain fatty acids (44% erucic acid, C, and 23% nervonic acid, C).
The oil is suitable as lubricant. In addition, recent developments indicate that nervonic acid may be used as raw material for the production of a medicine against multiple sclerosis. by Robert G. Franks North Carolina State University, Raleigh, NC., USA How organ size and shape are determined in developing organisms remains a key question of interest to developmental biologists.
An understanding of the relationships between gene expression and the organ shape requires analyses of relationships between the subcellular, cellular, and organ levels and is. methyl nitroso urea (MNH). In several cases of in vitro chemical mutagenesis, explants and calli are treated with MNNG (80 mg l–l), EMS (%), NaN 2 (M) and N 2H 2 (M) (Bourhamont and Dubin ).
EMS is generally used in a concentration range of to 1% whereas the range for nitroso-ethyl urea is to mM (Deane et al. Current Research on Micropropagation of Sulcorebutia Alba Rausch, Syringa Vulgaris L., Hippeastrum Hybrids and Tulipa Hybrids. Authors; R.L.M. Regeneration, vernalization and flowering in Lunaria annua L.
in vivo and in vitro. Meded. Landbouw Hogeschool Wageningen, The. ADVERTISEMENTS: In this article we will discuss about: 1. Definition of Vernalization 2.
Site of Vernalization 3. Nature of Vernalization Process 4. Application. Definition of Vernalization: The precise definition of vernalization is not universally accepted. The term is best defined as the specific promotion of flowering by a cold treatment given to the imbibed seed [ ].
The percentage of flowering reduced from 80 to 60% when the cultures were exposed to shorter day length. By adding mg L-1 NAA, the in vitro flowering was found to occur faster (21 days) (Table 1).
GA 3 was not effective for in vitro flowering of this species although it may be effective for other species. The leaves. Vernalization is an environmentally induced epigenetic switch in which winter cold triggers epigenetic silencing of floral repressors and thus provides competence to flower in spring.
Vernalization triggers the recruitment of chromatin-modifying complexes to a clade of flowering repressors that are epigenetically silenced via chromatin modifications.
serpentina to figure in the Red Data Book. In vitro biology and technology have been proposed for micropropagation of R. serpentina[7,8,9,10,11,12,13] as well as in R.
tetraphylla[14,15,16,17] using shoot tip, leaf, nodal and root explants. In the present paper in vitro plant regeneration. SRIVASTAVA et al: IN VITRO PLANT REGENERATION OF EPIPHYTIC A.
RINGENS at lower concentrations. The maximum seed germination rate (%±) was recorded in KC medium supplemented with μM BAP and mg L-1 peptone, followed by MS medium supplemented with μM BAP and mg L-1 peptone (±). Adventitious Root Formation in Isolated Petiole Segments of Lunaria annua L.
PIERIK With 2 figures Received Aug Zusammenfassung Die Adventivwurzelbildung bei in vitro kultivierten Blattstielsegmenten vegetativer Lunaria annua L. Pflanzen wurde durch das Alter des Blattstieles stark beeinflulk Nur Blattstiele der jiingsten Blatter konnten Adventivwurzeln regenerieren.
Methods are described for the vegetative propagation of Begonia venosa Skan. Young flower buds are capable of producing callus which, contrasting to callus from leaves of adult plants, is very organogenic. For callus induction are required: BA and NAA at a conc.
of mgl−1, 21 °C and low irradiance. Subculture of organogenic callus is optimal on a medium with mgl−1 BA and 2% glucose. Chang, Z.M. and Reisch, B.I. Shoot regeneration from petioles and leaves of Vitis x Labruscana “Catawba”, Plant Cell Rep. 8: Chee R, Pool R M. In vitro propagation of Vitis: The effect of organic substances on shoot multiplication.
Vitis Chee R, Pool R M. Improved Inorganic Media Constituents for in. Vernalization and gibberellins in Lunaria annuaL J.
L. Stoddartand Effects ofdaylength on gibberellin synthesis in leaves ofred clover (Trifolium pratense L.) Colette Nitsch. Effects of growth substances on the induction of flowering of a short-day plant in vitro Flowering activities of natural and chemical.Singh, et al, Somatic embryogenesis and in vitro regeneration of an endangered medicinal plant sarpgandha (Rauvolfia serpentina L.) callus was cut into small pieces transferred to MS media having BAP and IAA in same concentration as for cal-lus induction.
Subculturing was .Cytological Investigations on Roots Grown In Vivo versus In Vitro. One hundred () seeds of Celosia cristata were germinated on moist cotton wool in Petri dishes for 2 weeks, and also on MS basal medium (for comparison), to obtain standard growth curves for the primary roots.
The primary root length of the population was measured once per day at a fixed time and the mean root length of.